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hfcγriiia  (R&D Systems)


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    R&D Systems hfcγriiia
    Hfcγriiia, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hfc%CE%B3riiia/Recombinant+Human+Fc+gamma+RIIIA%2FCD16a+Protein%2C+CF/pm38527972-231-52-55
    Average 94 stars, based on 82 article reviews
    hfcγriiia - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Binding Assay:

    Article Title: RUBY® - a tetravalent (2+2) bispecific antibody format with excellent functionality and IgG-like stability, pharmacology and developability properties.
    Article Snippet: RUBY bsAb and monoclonal isotype controls were diluted to 200 nM in 1× Kinetic buffer and captured on 8 parallel sensors for 300 sec. After setting a new baseline, the captured antibodies were assayed against any of the FcγR (hFcγRI (1257-FC -050, R&D Systems), hFcγRIIa (1330-CD-050, R&D Systems), hFcγRIIb (1875-CD-050, R&D Systems), hFcγRIIIa (4325-FC -050, R&D Systems), hFcγRIIIa V176F (8894-FC-050, R&D Systems), mFcγRI (2074-FC-050, R&D Systems), mFcγRIIb (1460-CD-050, R&D Systems), mFcγRIII (1960-FC-050, R&D Systems) mFcγRIV (50036-M27H-50, Sino Biologicals) for 60 seconds followed by dissociation for 60 seconds in kinetic buffer.. The FcγRs were diluted in seven 1:2 dilutions starting at 100 nM.The FcγRs were diluted in seven 1:2 dilutions starting at 100 nM.

    Article Title: RUBY® – a tetravalent (2+2) bispecific antibody format with excellent functionality and IgG-like stability, pharmacology and developability properties
    Article Snippet: RUBY bsAb and monoclonal isotype controls were diluted to 200 nM in 1× Kinetic buffer and captured on 8 parallel sensors for 300 sec. After setting a new baseline, the captured antibodies were assayed against any of the FcγR (hFcγRI (1257-FC-050, R&D Systems), hFcγRIIa (1330-CD-050, R&D Systems), hFcγRIIb (1875-CD-050, R&D Systems), hFcγRIIIa (4325-FC-050, R&D Systems), hFcγRIIIa V176F (8894-FC-050, R&D Systems), mFcγRI (2074-FC-050, R&D Systems), mFcγRIIb (1460-CD-050, R&D Systems), mFcγRIII (1960-FC-050, R&D Systems) mFcγRIV (50036-M27H-50, Sino Biologicals) for 60 seconds followed by dissociation for 60 seconds in kinetic buffer.. The FcγRs were diluted in seven 1:2 dilutions starting at 100 nM.The FcγRs were diluted in seven 1:2 dilutions starting at 100 nM.



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    Influenza-seronegative cynomolgus macaques received either unadjuvanted H1 stem v2 or H1 stem v2 adjuvanted with SLA-LSQ containing SLA and QS-21 in a ratio of 5:2 μg, 10:25 μg, 25:25 μg or 50:50 μg, H1 stem v2 adjuvanted with AS01 B containing 50:50 μg MPL ® and QS-21 or Al(OH) 3 + NaCl ( N = 8–9) on week 0 and 8. a Neutralizing antibody titers were measured using a ppVNA against a panel of influenza A group 1 virus strains at week 12 (4 weeks post dose 2). The median neutralizing titers per group are visualized in a heatmap using binned neutralization titers. b Breadth-potency curves of individual animals per group. Breadth denotes the neutralization coverage of the panel of ppVNA influenza strains as shown in ( a ). Red line represents the mean curve per group. c AUC of each breadth-potency curve as shown in ( b ). d ADCC was quantified using a H1N1 A/California/07/09 HA-specific hFcγRIIIa reporter assay at week 12. Endpoint titers are shown for individual animals. Red horizontal bars indicate the group medians and open symbols indicate the AUC for the breadth of neutralization is 0 or that ADCC was not detected. Comparisons between the SLA-LSQ- and Al(OH) 3 + NaCl-vaccine groups with the AS01 B group were performed by a Wilcoxon rank sum test. Statistical differences are indicated by asterisks: * P < 0.05, ** P < 0.01, *** P < 0.001. Additional statistical comparisons are shown in Supplemental Tables and .

    Journal: NPJ Vaccines

    Article Title: Enhancing breadth and durability of humoral immune responses in non-human primates with an adjuvanted group 1 influenza hemagglutinin stem antigen

    doi: 10.1038/s41541-023-00772-1

    Figure Lengend Snippet: Influenza-seronegative cynomolgus macaques received either unadjuvanted H1 stem v2 or H1 stem v2 adjuvanted with SLA-LSQ containing SLA and QS-21 in a ratio of 5:2 μg, 10:25 μg, 25:25 μg or 50:50 μg, H1 stem v2 adjuvanted with AS01 B containing 50:50 μg MPL ® and QS-21 or Al(OH) 3 + NaCl ( N = 8–9) on week 0 and 8. a Neutralizing antibody titers were measured using a ppVNA against a panel of influenza A group 1 virus strains at week 12 (4 weeks post dose 2). The median neutralizing titers per group are visualized in a heatmap using binned neutralization titers. b Breadth-potency curves of individual animals per group. Breadth denotes the neutralization coverage of the panel of ppVNA influenza strains as shown in ( a ). Red line represents the mean curve per group. c AUC of each breadth-potency curve as shown in ( b ). d ADCC was quantified using a H1N1 A/California/07/09 HA-specific hFcγRIIIa reporter assay at week 12. Endpoint titers are shown for individual animals. Red horizontal bars indicate the group medians and open symbols indicate the AUC for the breadth of neutralization is 0 or that ADCC was not detected. Comparisons between the SLA-LSQ- and Al(OH) 3 + NaCl-vaccine groups with the AS01 B group were performed by a Wilcoxon rank sum test. Statistical differences are indicated by asterisks: * P < 0.05, ** P < 0.01, *** P < 0.001. Additional statistical comparisons are shown in Supplemental Tables and .

    Article Snippet: To measure HA-specific antibody-dependent cellular cytotoxicity (ADCC) Fc effector functions elicited by H1 stem vaccination, serum samples were analyzed using an ADCC hFcγRIIIa reporter assay (Promega).

    Techniques: Virus, Neutralization, Reporter Assay